The Prevalence of VIM, IMP, and NDM-۱ Metallo-beta-Lactamase Genes in Clinical Isolates of Klebsiella pneumoniae in Qom Province, Iran

Introduction: An increase in the consumption of antibiotics has raised significant concerns over the treatment of Klebsiella pneumonia-infected patients. In this study, the resistance pattern of K. pneumoniae to antibiotics such as imipenem, meropenem, and ertapenem, as well as the frequency of Metallo-beta-lactamase (MBL) genes, namely VIM, IMP, and NDM-۱ were investigated. Methods: Following the isolation of ۲۰۰ K. pneumoniae isolates from ۶۵۰ clinical samples, the antibiotic resistance pattern of these isolates against different antibiotics was evaluated. The isolates resistant to imipenem, meropenem, and ertapenem were identified, and the presence of VIM, IMP, and NDM-۱ genes was examined by using PCR methods. Results: The K. pneumoniae isolates exhibited different resistance patterns in response to various antibiotics. The frequency of VIM, IMP, and NDM-۱ genes showed that ۴۸ strains are resistant to imipenem, meropenem, and ertapenem in which ۱۵.۶% was positive for IMP, ۲.۴۲% for VIM, and ۱.۹۲% positive for NDM-۱ gene. The isolates showed the highest antibiotic resistance to ampicillin (۹۷.۵%) and the lowest to meropenem (۵.۵%). Conclusion: Considering carbapenem antibiotics such as imipenem, meropenem, and ertapenem which are known to be among the most frequently used antibiotics for the treatment of K. pneumoniae infections and the involvement of MBL genes in this scenario, we aimed to screen and identify MBL genes responsible for the resistance of K. pneumoniae to imipenem, meropenem, and ertapenem.

کلیدواژه ها:

Klebsiella pneumonia ، Metallo-beta-lactamase genes ، Antibiotic resistance ، PCR