Protective Effects of Hydroxytyrosol on Renal Ischemia-reperfusion Injury in Mice

Background: Renal ischemia reperfusion (IR), one of the most important causative mechanisms of acute kidney injury, is a major clinical problem that occurs in some hospitalized patients. The aim of this study was to investigate the effects of hydroxytyrosol (HT) on renal ischemia-reperfusion injury in mice.Methods: Male mice were randomly assigned to three groups (N = ۹): (۱) sham operated, (۲) renal IR (۴۵ min ischemia followed by ۲۴ h reperfusion), or (۳) renal IR+HT (۵ mg/kg orally for ۷ days before renal IR and one hour before IR). Animals were sacrificed and the blood and kidney tissue samples were collected for glutathione (GSH) and malondialdehyde (MDA) evaluation.Results: Renal IR injury led to decreases in renal tissue GSH and increases in MDA levels compared to sham operated. Hydroxytyrosol partially inhibited the IR-induced decrease in GSH activity. In addition, in the renal IR+HT group, MDA was increased compared to the renal IR group.Conclusions: These findings suggest that HT diminished oxidative stress in renal tissue after renal IR.Background: Renal ischemia reperfusion (IR), one of the most important causative mechanisms of acute kidney injury, is a major clinical problem that occurs in some hospitalized patients. The aim of this study was to investigate the effects of hydroxytyrosol (HT) on renal ischemia-reperfusion injury in mice. Methods: Male mice were randomly assigned to three groups (N = ۹): (۱) sham operated, (۲) renal IR (۴۵ min ischemia followed by ۲۴ h reperfusion), or (۳) renal IR+HT (۵ mg/kg orally for ۷ days before renal IR and one hour before IR). Animals were sacrificed and the blood and kidney tissue samples were collected for glutathione (GSH) and malondialdehyde (MDA) evaluation. Results: Renal IR injury led to decreases in renal tissue GSH and increases in MDA levels compared to sham operated. Hydroxytyrosol partially inhibited the IR-induced decrease in GSH activity. In addition, in the renal IR+HT group, MDA was increased compared to the renal IR group. Conclusions: These findings suggest that HT diminished oxidative stress in renal tissue after renal IR.

کلیدواژه ها:

Ischemia reperfusion ، Acute kidney injury ، Hydroxytyrosol ، Oxidative stress.