Characterization of Staphylococcus aureus Isolated From Wound Infectious in Diabetes Clinic of Hazrat Fatemeh Zahra (SA) Hospital

Background: Staphylococcus aureus is a gram positive pathogen which causes a wide range of infections. The present study aimed to investigate genotypic and phenotypic screening about biofilm formation in S. aureus isolated from wound infections in a diabetes clinic in Hazrat Fatemeh Zahra (SA) hospital. Methods: A total of ۲۶۷ clinical samples were collected from various types of wound infections in the diabetes clinic of Hazrat Fatemeh Zahra (SA) hospital, Isfahan, Iran. The methicillin-resistant S. aureus (MRSA) isolates were selected and biofilm formation and its related genes were analyzed by polymerase chain reaction (PCR). Results: The results showed that ۹۵ out of ۱۳۲ samples were MRSA. The high resistance was seen to methicillin, erythromycin, ciprofloxacin, and penicillin. Phenotypic results showed that ۴۸.۳% of the isolates were high biofilm producers, ۲۹.۱% were average biofilm producers, and ۱۰.۶% were low biofilm producers. Conclusions: According to the results of this study, the expression levels of biofilm-associated genes significantly increased, and high prevalence of antibiotic resistance was one of the important reasons for the development of drug resistance in patients.