Molecular Rapid Diagnostic Test for Nosocomial Nonfermenting Gram-Negative Bacilli

Accurate diagnosis of nosocomial infections is crucial for appropriate antibiotic therapy, as well as avoiding unnecessary use of drugs. Mainly ۳ gram-negative bacteria including Pseudomonas aeruginosa, Acinetobacter baumannii, and Stenotrophomonas maltophilia are involved in nosocomial infections. These bacteria are possibly misdiagnosed with together in traditional diagnostic methods. In this regard, a rapid molecular diagnostic kit was developed in Shiraz Burn Research Center. Using this diagnostic kit, the sensitivity and specificity analyses showed following results, respectively: P. aeruginosa: ۱۰۰%, ۹۶.۸%; A. baumannii: ۱۰۰%, ۱۰۰%, and S. maltophilia: ۹۶.۷%, and ۹۳.۸%. Therefore, this multiplex polymerase chain reaction (PCR) method with ۳ primer sequences could be a responsive technique in timely and appropriate detection of studied bacteria.