Determination of Cyproheptadine-Hydrochloride by Precipitation with ۳, ۵-Dintrosalicylic Acid via the NAG-۴SX۳-۳D Analyzer at ۰-۱۸۰˚

This study utilized the NAG-۴SX۳-۳D analyzer (four sources of white snow light-emitting diodes arranged in three rows corresponding to three detectors), a tool for optical, chemical, electronic, and detection based on receiving cumulative signals (no amplification is required). Total travelling distance is ۷۶۰ mm with regard to YZ(mV)–t sec (d mm). It was selected for its precise calculation of the energy transducer profile (NAG۴X۳) to accurately calculate the energy output detector. There was a goal to help scholars understand the technology better by giving them accurate information about the device. The technology provides a low-cost, efficient, precise, and responsive method. CPH in pharmaceutical samples has been determined using the turbidity technique. This procedure does not require the use of any extra materials. CPH is predicted to react immediately with ۳,۵-DNSA, resulting in the creation of a bright yellow precipitate. Cyproheptadine has a linear range of ۰.۰۰۵ to ۴۰ mM. The relative standard deviation (RSD%) for the n = ۶ at ۱۰ mM and ۱۵ mM doses was substantially less than ۰.۱۵%. Starting from the lowest concentration on the calibration curve and gradually diluting it, the limit of detection (LOD) was found to be ۱۶۳.۹۵۸ ng per analyte, and the limit of quantification (LOQ) was found to be ۲۴۵.۶۳۲ μg/sample. The linear dynamic range has a strong correlation value (r = ۰.۹۹۸۶) and a high percentage of linearity (R۲% = ۹۹.۷۲), with recovery not less than ۹۵.۷۸%. The findings of the newly created approach were found to be similar to those of the previous technique (UV-spectrophotometric at a wavelength of ۲۷۴ nm). The suggested method demonstrates enhanced sensitivity, utilizes a reduced quantity of chemicals, and yields superior outcomes compared to the reference approach, which involves ۱۰ mm irradiation.

کلیدواژه ها:

NAG-۴SX۳-۳D analyzer ، Cyprohetadine-Hydrochloride ، ۳ ، ۵-Dinitrosalicylic acid