Enhancing haploid wheat induction efficiency through the wheat × maize crossbreeding System utilizing silver nitrate and calcium phosphate

This study aimed to assess the efficacy of silver nitrate and calcium phosphate in inducing chromosome elimination and promoting haploid wheat generation in the context of wheat-maize crosses. The experiment explored the impact of various treatments, including non-use (control) and the utilization of silver nitrate (۳gr/l) combined with calcium phosphate (۰.۱gr/l). Additionally, different durations of treatments post-pollination, spanning ۴۸ and ۷۲ hours, were considered. The study evaluated key characteristics such a number florets, number as well as the quantity and percentage of successful seed formations. The findings underscored significant distinctions among the treatments, achieving a level of significance of ۱%. The use of silver nitrate in conjunctionwith calcium phosphate for wheat tillers demonstrated an increased propensity for haploid embryo formationSpecifically, maintaining wheat tillers in a solution of silver nitrate and calcium phosphate for ۷۲ and ۴۸ hours, in comparison to the control group, resulted in haploid embryo formations with proportions of ۱۲.۵۳%, ۵.۶۰%, and ۳.۸۲%, respectively. These results indicate that extended exposure to the silver nitrate-calcium phosphate treatmentenhances the formation of haploid embryos.

کلیدواژه ها:

Haploid ، Chromosome elimination ، Interspecific hybridization ، Embryo rescue ، tissue culture