Effect of Home-based Aerobic Exercise on Leukocyte TERT Gene Expression Without Altering DNA Oxidative Damage in Postmenopausal Women
محل انتشار: فصلنامه پزشکی هرمزگان، دوره: 27، شماره: 4
سال انتشار: 1402
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 89
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شناسه ملی سند علمی:
JR_HMJ-27-4_008
تاریخ نمایه سازی: 3 بهمن 1402
چکیده مقاله:
Objectives: In middle-aged women, the postmenopausal period is associated with increased oxidative, inflammatory, and psychological stress. We intend to investigate the effect of home-based aerobic exercise (HAE) on serum levels of ۸-hydroxy-۲’-deoxyguanosine (۸-C) and the expression of telomerase reverse transcriptase (TERT) in leukocytes of postmenopausal women.
Methods: A total of ۲۰ sedentary postmenopausal women who had at least ۱۲ months passed since their last menstruation participated in the study voluntarily. Their Mean±SD age, height, weight, and body mass index (BMI) were respectively ۵۲.۴±۳.۸ years, ۱۶۱.۴±۳.۷ cm, ۷۴.۴±۶.۸ kg, and ۲۸.۶±۳.۴ kg/m۲. They were randomly divided into two groups: Home-based aerobic exercise (HAE, n=۱۰) and inactive control (CON, n=۱۰). HAE group undertook ۴ weeks of home-based aerobic exercise (۴ d/wk, intensity: ۷۰%-۸۰% maximal heart rate) with online supervision. Participants’ weight and fasting blood samples (۱۲-h fasting state) were measured and taken before and ۴۸ hours after the intervention. TERT expression in blood leukocytes and serum levels of ۸-OHDG were assessed by real-time PCR and ELISA methods, respectively. The collected data were analyzed using mixed (۲×۲) repeated measure ANOVA and independent samples t-test in SPSS software. A significance level of P<۰.۰۵ was considered for all tests.
Results: The findings showed that ۴ weeks of HAE increased TERT expression significantly compared to the CON (ES=۰.۴۰, P<۰.۰۵). Although ۸-OHDG serum level in the HAE group increased following training compared to pre-training (P<۰.۰۵), no significant difference was observed between the CON and HAE groups (P>۰.۰۵). Also, the interaction effect on ۸-OHDG was not significant (ES=۰.۱۱, P>۰.۰۵). In addition, after ۴ weeks of HAE, body weight and BMI significantly decreased in the training group compared to the control (P<۰.۰۵).
Discussion: According to these findings, HEA effectively reduces telomere erosion and improves weight control in postmenopausal women.
کلیدواژه ها:
نویسندگان
Fahime Khairabadi
Department of Exercise Physiology, Faculty of Sport Sciences, University of Guilan, Guilan, Iran.
Abbas Ali Gaeini
Department of Exercise Physiology, Faculty of Sport Sciences, University of Tehran, Tehran, Iran.
Alireza Niknam
Department of Sport Sciences, Faculty of Psychology and Educational Sciences, University of Shiraz, Shiraz, Iran.
Maryam Koushkie Jahromi
Department of Sport Sciences, Faculty of Psychology and Educational Sciences, University of Shiraz, Shiraz, Iran.
Mohammadreza Kordi
Department of Exercise Physiology, Faculty of Sport Sciences, University of Tehran, Tehran, Iran.