Fabrication and Characterization of Thymus Daenensis Essential Oil and Glycyrrhiza Glabra L. Extract Loaded Core-shell Nanofiber Scaffolds for Wound Healing

Since ancient times, the wound healing properties of herbal medicines have been of interest and plant extracts have been traditionally used for such applications. In this study, A core-shell nanofiber scaffolds of PVA and Gelatin was designed by electrospinning technique that Thymus daenensis essential oil (۵,۱۰ and ۲۰%), as a natural antimicrobial agent, and Glycyrrhiza glabra L. extract (۲,۴ and ۸%) as wound healing agent were incorporated into core and shell of nanofiber scaffolds respectively. The electrospun were done successfully and all samples showed bead-free and tubular nano-fibers with no phase breakdown in the structure. The diameter of the nanofibers with adding essential oils and extracts in different concentrations, between ۲۱۵.۹۹۹ and ۲۸۰.۸۹۳ have been prepared. Presence of Thymus essential oil and Glycyrrhiza glabra L. extract in the electrospun fibers was approved by Fourier Transform Infrared spectroscopy. Incorporation of Thymus essential oil and Glycyrrhiza glabra L. extract into the nanofiber mats exhibited significant synergistic antibacterial activity against bacterial cells. Cell viability and proliferation were confirmed for all fabricated nanofibers on L۹۲۹ cells. All concentrations of T. daenensis essential oil and G. glabra extract in nanofibers had positive effect on fibroblast migration in the scratch test, which showed that this fibroblast migration causes skin regeneration and production of new granulation tissue and faster wound healing. Therefore, the results of this work showed that Thymus essential oil and Glycyrrhiza glabra L. extract incorporated core shell nanofibers have a great potential to be used as the biodegradable, biobased and antibacterial wound healing dressings.

کلیدواژه ها:

Electrospinning ، core-shell nanofiber ، electrospinning ، Thymus daenensis ، Glycyrrhiza glabra