Lab Scale Production of Recombinant Platelet-derived Growth Factor-BB Homodimer (PDGF-BB) in Microbial Expression System for Wound Healing Applications

Skin wound healing depends on different and overlapping phases including hemostasis, inflammation, proliferation, and dissolution/remodeling. Wound healing will be delayed by any blockage period, which often enters the wound into a state of pathological inflammation. Recombinant proteins are proteins encoded by recombinant DNA technology. In recent years, the number of recombinant proteins used for therapeutic applications has increased dramatically. Among the growth factors, platelet-derived growth factors (PDGFs) are effective in the management of injury and involve many cellular events in the healing process, including inflammatory cell recruitment, fibroblast proliferation and migration, intraepithelial collagen deposition, and granulation tissue formation. During the process of natural wound healing, platelets are one of the first cells that respond at the wound site or around it and play an essential role in wound healing. Platelets are a rich source of growth factors, including PDGF. PDGF is the first and only recombinant growth factor approved by the US Food and Drug Administration (FDA) for topical administration to treat diabetic foot ulcers.Methods: In this study, we’ve built a microbial expression vector containing the coding sequence for optimized PDGF-BB and transformed it into an E. coli Strain. Then We developed a complete production process that includes, expression, purification, and biological activity assay.Results: The purified PDGF-BB (۵۰۰ μg⁄L of culture) was well-folded and biologically active. The whole procedure is fast and scalable.Conclusions: Chosen E. coli Strain can produce and fold recombinant PDGF-BB. The produced PDGF-BB has biologically active, cost benefits and could be considered for further investment and investigation.

کلیدواژه ها:

Recombinant PDGF-BB ، Recombinant DNA Technology ، Microbial Expression System ، Wound Healing