Resveratrol - Grafted Cellulose Scaffolds: A Novel Approach for Enhancing Tissue Repair

Introduction: Nanobiotechnology has developed as a promising field for the development of novel therapeutic strategies to address the challenges associated with tissue repair and regeneration. Over the past few decades, tissue engineering has garnered significant interest because of its capacity to innovate advanced biomaterials, replicate healthy tissue settings, and contribute to the creation of cutting-edge delivery systems for tissue construction, repair, and regeneration. Its pivotal role in restoring or establishing normal function in human cells, tissues, and organs is paramount in the field of regenerative medicine. In recent years, the combination of natural compounds and nano-materials has emerged as a novel approach to enhance tissue repair strategies. Resveratrol, a small molecule renowned for its regenerative properties, has shown great potential in various tissue repair applications.Purpose: The aim of our study is to investigate the potential of resveratrol-grafted cellulose scaffolds as a novel strategy for enhancing dermal tissue repair. By grafting resveratrol onto cellulose scaffolds, we aim to synergistically harness the benefits of both the natural compound and the biomaterial. The specific purposes of this study are to evaluate the grafting technique, assess the controlled release profile of resveratrol from the scaffold, and investigate the biological responses and potential implications for tissue regeneration.Materials and methods: For preparation of scaffold, we utilized the endocarp of Phoenix dactylifera fruit, SDS employed in endocarp decellularization, MTT assay employed to determine resveratrol's cytotoxicity concentration; to achieve optimal stability, the coated small molecule underwent Freeze-drying, preserving its integrity on the scaffold, to investigate cellular treatment, we utilized the NIH ۳T۳ fibroblast cell line and employed Flow-cytometry for quantitative assessment of cell growth and proliferation.Results: The release profile of resveratrol from the scaffold manifested sustained release over a specific time period, indicating its potential for localized delivery. In vitro studies using the NIH ۳T۳ fibroblast cell line showed enhanced cell growth and proliferation on the resveratrol-grafted scaffolds compared to control one. Flow-cytometry analysis revealed a significant increase in cell viability and proliferation rates. These findings indicate the potential of resveratrol-grafted cellulose scaffolds as a promising strategy for enhancing dermal tissue repair and regeneration.

کلیدواژه ها:

Nano-material ، Dermal tissue repair ، Natural scaffold ، Tissue engineering