Effect of Exposure to ۹۰۰ MHz GSM Mobile Phone Radiofrequency Radiation on Estrogen Receptor Methylation Status in Colon Cells of Male Sprague Dawley Rats
محل انتشار: مجله فیزیک و مهندسی پزشکی، دوره: 7، شماره: 1
سال انتشار: 1396
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 67
فایل این مقاله در 8 صفحه با فرمت PDF قابل دریافت می باشد
- صدور گواهی نمایه سازی
- من نویسنده این مقاله هستم
استخراج به نرم افزارهای پژوهشی:
شناسه ملی سند علمی:
JR_JBPE-7-1_009
تاریخ نمایه سازی: 30 دی 1402
چکیده مقاله:
Background: Over the past several years, the rapidly increasing use of mobile phones has raised global concerns about the biological effects of exposure to radiofrequency (RF) radiation. Numerous studies have shown that exposure to electromagnetic fields (EMFs) can be associated with effects on the nervous, endocrine, immune, cardiovascular, hematopoietic and ocular systems. In spite of genetic diversity, the onset and progression of cancer can be controlled by epigenetic mechanisms such as gene promoter methylation. There are extensive studies on the epigenetic changes of the tumor suppressor genes as well as the identification of methylation biomarkers in colorectal cancer. Some studies have revealed that genetic changes can be induced by exposure to RF radiation. However, whether or not RF radiation is capable of inducing epigenetic alteration has not been clarified yet. To date, no study has been conducted on the effect of radiation on epigenetic alterations in colorectal cancer (CRC). Several studies have also shown that methylation of estrogen receptor α (ERα), MYOD, MGMT, SFRP۲ and P۱۶ play an important role in CRC. It can be hypothesized that RF exposure can be a reason for the high incidence of CRC in Iran. This study aimed to investigate whether epigenetic pattern of ERα is susceptible to RF radiation and if RF radiation can induce radioadaptive response as epigenetic changes after receiving the challenge dose (γ-ray).Material and Method: ۴۰ male Sprague-Dawley rats were divided into ۴ equal groups (Group I: exposure to RF radiation of a GSM cell phone for ۴ hours and sacrificed after ۲۴ hours; Group II: RF exposure for ۴ hours, exposure to Co-۶۰ gamma radiation (۳ Gy) after ۲۴ hours and sacrificed after ۷۲ hrs; Group III: only ۳Gy gamma radiation; Group ۴: control group). DNA from colon tissues was extracted to evaluate the methylation status by methylation specific PCR.Results: Our finding showed that exposure to GSM cell phone RF radiation was capable of altering the pattern of ERα gene methylation compared to that of non-exposed controls. Furthermore, no adaptive response phenomenon was induced in the pattern of ERα gene methylation after exposure to the challenging dose of Co-۶۰ γ-rays. Conclusion: It can be concluded that exposure to RF radiation emitted by GSM mobile phones can lead to epigenetic detrimental changes in ERα promoter methylation pattern.
کلیدواژه ها:
نویسندگان
P Mokarram
Department of Biochemistry, Shiraz University of Medical Sciences, Shiraz, Iran
M Sheikhi
Department of Radiobiology, School of Paramedical Sciences, Shiraz, Iran
S M J Mortazavi
Ionizing and Non-ionizing Radiation Protection Research Center (INIRPRC), Shiraz University of Medical Sciences, Shiraz, Iran
S Saeb
Department of clinical biochemistry, school of medicine, Shiraz University of Medical Sciences, Shiraz, Iran
N Shokrpour
Professor, School of Paramedical Sciences, Shiraz University of Medical Sciences, Shiraz, Iran
مراجع و منابع این مقاله:
لیست زیر مراجع و منابع استفاده شده در این مقاله را نمایش می دهد. این مراجع به صورت کاملا ماشینی و بر اساس هوش مصنوعی استخراج شده اند و لذا ممکن است دارای اشکالاتی باشند که به مرور زمان دقت استخراج این محتوا افزایش می یابد. مراجعی که مقالات مربوط به آنها در سیویلیکا نمایه شده و پیدا شده اند، به خود مقاله لینک شده اند :