Association of FCγRIIA (CD۳۲) polymorphism with susceptibility to brucellosis

Background: Brucellosis is the major bacterial zoonoses of global importance caused by Brucella spps. FCγRIIA receptor plays a central role in phagocytosis of IgG۲-opsonized bacteria. FCγRIIA exhibits allelic polymorphisms with different capacities for binding IgG۲ and phagocytosis. Cells expressing Fc γ RIIa-H۱۳۱, bind more efficiently to complexes of IgG۲ than those expressing the Fc γ RII A -R۱۳۱ variant. The purpose of this study was to evaluate the association of FCγRIIA polymorphisms with susceptibility to or severity of brucellosis. Materials and Methods: In this study, we evaluated FCγRIIA polymorphisms (R/R۱۳۱, R/H۱۳۱, H/H۱۳۱) in ۶۷ patients with brucellosis and ۶۷ age, sex and geographical matched healthy volunteers. FCγRIIA genotyping was performed by using a sequence-specific primer polymerase chain reaction (SSP-PCR). Results: The comparison of the FCγRIIA genotypes distribution in patients with brucellosis and controls showed a higher frequency in FCγRIIA-R/R۱۳۱ homozygosity in patients than controls (۴۷.۸% vs. ۲۸.۴%). Logistic regression analysis showed that there is a significant correlation between R/R۱۳۱ genotype and brucellosis (OR=۲.۳, ۹۵%CI=۱.۳-۴.۲, P=۰.۰۴). Although the frequency of the FCγRIIA-R/R۱۳۱ was higher in patients with chronic brucellosis compared with acute brucellosis, we did not find any statistically significant differences (۵۳.۸% vs. ۴۶.۳%, P=۰.۶۵). Conclusion: The result of this study showed that the homozygous genotype of FCγRIIA-R/R۱۳۱ in patients with brucellosis may be associated with susceptibility to brucellosis as a genetic risk factor.

کلیدواژه ها:

Brucellosis ، FCγRIIA ، Polymorphism