Synergistic effect of Achillea millefolium L. combined with bleomycin on prostate cancer cell

Background: The aim of this study was to investigate the effect of methanolic extract of Achillea millefolium L. (MEA) on the antiproliferative activity of bleomycin on human prostate cancer and normal skin cells. Materials and Methods: Human prostate cancer cell (DU-۱۴۵) and human non-malignant fibroblast cell (HFFF۲) were treated with MEA at various concentrations ( ۲۰, ۱۰۰, ۵۰۰, ۱۰۰۰ and ۲۰۰۰ µg/ml), bleomycin alone and with their combinations. Further their MEA and bleomycin effects on cell viability were evaluated. Free radical scavenging property was determined for this herbal extract. Results: The combination of Achillea millefolium with bleomycin increased significantly inhibition of cell growth in cancer cell. MEA enhanced significantly cytotoxicity induced by bleomycin with ۶۰% and ۴۹% in survival rate at doses ۱۰۰۰ and ۲۰۰۰ µg/ml, respectively, while it was ۸۵% in bleomycin-treated cells. MEA did not exhibit any cytotoxicity on HFFF۲ cells. Conclusion: Study suggests that Achillea millefolium enhanced the cell toxicity induced by bleomycin in the prostate cancer cell without any significant toxicity on normal cell.

کلیدواژه ها:

Achillea millefolium ، prostate cancer ، Antiproliferative ، MTT ، antioxidantbleomycin