Frequencies of two CYP۲C۱۹ defective alleles (CYP۲C۱۹*۲, and *۳) among Iranian population in Mazandaran Province
محل انتشار: مجله تحقیق در پزشکی مولکولی، دوره: 1، شماره: 1
سال انتشار: 1391
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 74
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شناسه ملی سند علمی:
JR_REMJ-1-1_003
تاریخ نمایه سازی: 22 دی 1402
چکیده مقاله:
Background: Cytochrome P۴۵۰ ۲C۱۹ (CYP۲C۱۹) is a polymorphically expressed enzyme that shows marked interindividual and interethnic variation. CYP۲C۱۹*۲ and CYP۲C۱۹*۳ are the most frequently identified defective alleles in Orientals and Caucasian poor metabolizers (PM). The aim of this study was to investigate the frequencies of CYP۲C۱۹*۱, CYP۲C۱۹*۲ and CYP۲C۱۹*۳ alleles and CYP۲C۱۹ genotypes in the Mazandarani ethnic group among Iranian Population.
Materials and Methods: The study was conducted in ۱۰۳ unrelated healthy volunteers. DNA was extracted from leucocytes and analyzed by the PCR-RFLP protocol. The PCR product was digested with restriction enzymes (SmaI and BamH۱) and then separated electrophoretically using polyacrylamide gel..
Results: Of the alleles tested, CYP۲C۱۹*۱, and CYP۲C۱۹*۲, but not CYP۲C۱۹*۳, were detected. The frequencies for CYP۲C۱۹ alleles *۱, *۲, and *۳ were ۹۱%, ۹.۰%, and ۰.۰%, respectively. The frequencies of CYP۲C۱۹ genotypes *۱/*۱, *۱/*۲, *۱/*۳, *۲/*۲, *۲/*۳ and *۳/*۳ were ۸۴%, ۱۴%, ۰.۰%, ۲.۰%, and ۰.۰%, respectively.
Conclusion: The result of the present study showed that the two inactive alleles of CYP۲C۱۹ accounted for ۹.۰% of CYP۲C۱۹ alleles in our sample versus ۸.۸ - ۴۰.۱% reported in other populations. The frequencies of the studied alleles resulted significant differences between our sample and African and Eastern Asian populations.
کلیدواژه ها:
نویسندگان
Naghi Shahabi-Majd
Molecular and Cell Biology Research Centre
Mohammad bagher Hashemi-Sotehoh
Molecular and Cell Biology Research Centre
Baharak Habashi
Psychiatry and Behavioral Sciences Research Centre
Mohammad reza Shiran
Psychiatry and Behavioral Sciences Research Centre