Supplementation of Freezing Media with Cyclic Adenosine Monophosphate Analog and Isobutylmethylxanthine on Sperm Quality

Background: This study aimed to explore whether the addition of a cyclic adenosine monophosphate (cAMP) analog and isobutylmethylxanthine (IBMX) in freezing media improved sperm quality and what role cAMP has in this recovery. Materials and methods: ach semen sample was cryopreserved into four groups: fresh semen sample, as a control group, freezing medium + ۲.۵ mM cAMP analog and ۰.۲ mM IBMX, freezing medium + ۱۲.۵ mM cAMP analog and ۰.۲ mM IBMX, and freezing medium + ۲۵ mM cAMP analog and ۰.۲ mM IBMX. Sperm parameters after post-thaw were analyzed according to WHO instruction (۲۰۱۰). Viability, acrosome reaction, and DNA damage levels of the samples were evaluated. Results: Our results indicated that the effective concentrations of ۱۲.۵ and ۲۵ mM cAMP analog and ۰.۲ mM IBMX significantly improved the total motility, progressive motility, and viability of the frozen-thawed (P<۰.۰۵). However, non-progressive motility and immotile were significantly reduced in the ۱۲.۵ and ۲۵ mM cAMP analogs and ۰.۲ mM IBMX groups after thawing (P<۰.۰۵). During freezing the spermatozoa, the high concentration of the cAMP analog increased acrosome reaction after thawing in the ۲۵ mM and ۰.۲ mM IBMX treated samples (P<۰.۰۵). DNA fragmentation in ۲۵ mM cAMP analog and ۰.۲ mM (IBMX) supplementation was significantly lower compared to the other groups (P<۰.۰۵). Conclusions: Our findings revealed that in vitro cAMP analog and IBMX supplementation in freezing media play an important role in preventing cryodamage by maintaining the sperm functional parameters.

کلیدواژه ها:

cAMP analog (IBMX) ، cryopreservation ، Asthenozoospermic ، sperm quality