Diversity of Antibiotic-resistant of Tentative Motile Aeromonas Species Isolated From Clarias gariepinus (Burchell ۱۸۲۲) Cultured in Earthen Ponds

Background: Aeromonas species is one of the most important causes of diseases in Clarias gariepinus, a public health threat with significant economic losses. Objectives: In this research, the prevalence and variety of Aeromonas species isolated from C. gariepinus cultured in an earthen pond were investigated, as well as the antibiogram and multiple antibiotic resistance indexMethods: Aeromonas species were isolated by culture and biochemical test and confirmed using a Microbact ۲۴E kit. The antibiotic susceptibility to ۱۰ antibiotics was determined using the Kirby-Bauer disk diffusion method.Results: Aeromonas species were isolated with a prevalence of ۴۳.۱% with ۴ different phenospecies with the highest prevalence of ۴۶(۲۴ %) for Aeromonas hydrophila followed by ۱۵(۷.۸%) for Aeromonas caviae, then ۱۰(۵.۲%) for Aeromonas veronii sobria, and the least was observed for Aeromonas veronii veronii ۶(۳.۱%). The Aeromonas species showed high resistance to amoxicillin, ampicillin, colistin sulfate, oxytetracycline, trimethoprim/sulfamethoxazole, and penicillin, with varying resistant patterns, and the multiple antibiotic resistance index values ranged between ۰.۲۰ and ۰.۸۰. Conclusion: There was a diversity of Aeromonas species associated with multiple antibiotic-resistant leading to the wide spread of antimicrobial resistance. Therefore, there is a need to control the use of antibiotics and ensure the effective use of biosecurity and preventive management measures in fish farms.

کلیدواژه ها:

antibiogram ، Fish farm ، Freshwater fish ، Multiple antibiotic resistance ، zoonosis