Method validation of clonidine hydrochloride in human plasma by LC-MS technique

A simple and sensitive high performance liquid chromatography-electrospray ionization mass spectrometry method has been evaluated for the assignment of clonidine hydrochloride in human plasma. The mobile phase composed of acetonitrile–water ۶۰:۴۰ (v/v), and ۰.۲% formic acid ۲۰ µL of sample was chromatographically analyzed using a repacked ZORBAX-XDB-ODS C۱۸ column (۲.۱ mm×۳۰ mm, ۳.۵ micron). Detection of analytes was achieved by tandem mass spectrometry with electrospray ionization (ESI) interface in positive ion mode was operated under the multiple-reaction monitoring mode (m/z ۲۳۰.۰ → ۲۱۳). Sample pretreatment involved in a one-step protein precipitation (PPT) with methanol and percholoric acid (HClO۴) of ۰.۱۵ mL plasma. Standard curve was linear (r = ۰.۹۹۸) over the concentration range of ۰.۰۱-۱۰.۰ ng/ml and showed suitable accuracy and precision. The limit of quantification (LOQ) was ۰.۰۱ ng/ml. The method is rapid, simple, very steady and precise for the separation, assignment, evaluation of clonidine healthy in human plasma.

کلیدواژه ها:

Clonidine hydrochloride ، LC-MS ، Human plasma