Mapping of TP۵۳ protein network using cytoscape software

TP۵۳ acts as a tumor suppressor in cancer. It induces cell cycle arrest or apoptosis in response to cellular stress and damage. p۵۳ gene alteration could cause uncontrolled cell proliferation.In the present study, we used TP۵۳ gene as the seed in the construction of a protein-protein functional association network to identify genes that might involve in tumorgenesis process with TP۵۳. TP۵۳ protein interaction database was obtained from STRING version ۹.۱ program. High-throughput experimental data, literature data and hypothetical studies have been used to determine the roles of candidate genes in TP۵۳ pathway. A total ۵۰۰ genes from STRING database loaded into Cytoscape version ۲.۸.۳. The ۱۷۶۲ protein interactions were assembled and visualized in y organic form. We found eight specific non-overlapping clusters of various sizes, which emerged from the huge network of protein-interactors using MCODE version ۱.۳۲ clustering algorithm. Biological Networks Gene Ontology (BiNGO) was used to determine two ontologies (molecular function and biological process) involved in the protein network. Most of the genes mainly participated in gene and protein expression, cell signaling and metabolism. A better understanding of the relationship between the genes could aid in developing prognostic markers and better therapeutic strategies in cancer treatment.

کلیدواژه ها:

TP۵۳ ، STRING ، MCODE clustering ، BiNGO