Evaluation of Susceptibility and Resistance of Human Infectious Bacteria and Identification of Bioactive Compounds in Pistacia atlantica, Cassia absus, and Quercus persica

Background: The antimicrobial activity of plants has long been considered an effective mechanism for controlling pathogenic microorganisms. Objectives: This study aimed to identify phytochemical compounds of the seed extracts from ethnomedicinal plants of Pistacia atlantica, Cassia absus, and Quercus persica with Gas Chromatography-Mass Spectrometry (GC-MS) and investigation of their antibacterial and antioxidant activities. Methods: The seeds were collected from Lorestan Province, Iran. Their antibacterial and anti-radical activities were analyzed by disk-diffusion and ۲,۲-diphenyl-۱-picrylhydrazyl assays, respectively. Ethanol (۹۶%), methanol (۸۰%), and distilled water extracts were obtained by the maceration method. The methanol extract was used for the analysis of chemical compositions. Results: About ۴۰, ۳۱, and ۸ compounds were identified by GC-MS in the seeds of C. absus, P. atlantica, and Q. persica, respectively. Results indicated that ۲,۴-di-tert-butylphenol (۳۶.۰۴۳%) and tetradecanoic acid (۴.۹۲%) were dominated in the seed extracts of C. absus. However, germacyclopetene (۳۸.۱۱۹%) and ۱,۲,۳-benzenetriol (۸.۱۱۵%) were dominated in the seed extracts of P. atlantica. Furthermore, ۵H-tetrazole-۵-thione, ۱,۴-dihydro-۱,۴-dimethy (۳۸.۵۰۵%), and tetradecanoic acid (۳۰.۵۴۶%) were dominated in the seed extracts of Q. persica. The highest inhibitory activity against Micrococcus luteus was observed on the methanol extract of C. absus with ascorbic acid. A significant difference was observed between the Inhibitory Concentration (IC۵۰) values of methanol extract of C. absus with ascorbic acid. Conclusion: Because of the presence of antimicrobial compounds in the tested ethnomedicinal plants, they can be used to synthesize new antimicrobial drugs in medicinal and pharmaceutical sciences.

کلیدواژه ها:

Gas Chromatography-Mass Spectrometry (GC/MS) ، Cassia absus ، Pistacia atlantica ، Quercus persica ، Infectious bacteria