The Cytotoxic Effect of the Tarragon (Artemisia dracunculus L.) Hydroalcoholic Extract on the HT-۲۹, MKN۴۵, and MCF-۷ Cell Lines

Background::Cancer is one of the problems facing societies today and despite new advances in chemotherapy and cancer treatment, there are still many cancers that do not respond to today’s treatments. Tarragon with the scientific name of Artemisia dracunculus L. has various flavonoid and polyphenolic compounds and many therapeutic effects.  Objectives: This study aimed to investigate the cytotoxicity of this plant on different categories of cancer cels. Methodes: After collecting the shoots of tarragon and extracting them by the maceration method, the weight of the extract with a yield of ۲۲.۲۵% was ۱۲.۹ g. After examining the presence of flavonoids and total phenol, the extract’s antioxidant activity was examined using DPPH and FRAP methods. Finally, MTT tests on three cancer cell lines, MCF-۷, HT-۲۹, and MKN۴۵, were done using different concentrations of tarragon extract (۱۰۰, ۲۰۰, ۵۰۰, and ۱۰۰۰ μg/mL). Results: Total flavonoids were detected at ۲۴±۱.۱۸ mg of quercetin per gram of extract, and total phenols were detected at ۵۹±۲.۲۱ mg of gallic acid per gram of extract. Examining the inhibitory effect of DPPH compared to vitamin C, it was found that the hydroalcoholic extract of tarragon has a ۵۰% inhibitory effect. According to the standard curve, the amount of iron reduced by tarragon hydroalcoholic extract is equal to ۴۰۵±۰.۱۱ μg/ml. The cytotoxic effect of tarragon hydroalcoholic extract on MCF۷, MKN۴۵, and HT-۲۹ cell lines was investigated, and their IC۵۰ values were ۱۰۶۵.۶۶۹, ۸۸۱.۱۹, and ۷۴۳.۸۷۰ μg/mL, respectively. The A. dracunculus L extract inhibits the growth of cancer cells in various cell lines.  Conclusion: According to antioxidant tests, it can be said that the anti-cancer effects of tarragon are based on its antioxidant power and phenolic and flavonoid compounds.

کلیدواژه ها:

Cytotoxicity ، Cancer ، Tarragon ، Artemisia dracunculus L ، Cell line