Evaluation of Antimicrobial Resistance in the Beta-lactamase Producing Escherichia Coli Isolated from Urinary Tract Infection in the Patients Referring to Taleghani Hospital of Tehran

Background & Objectives: Urinary tract infection is a common disease, especially among women. Beta-lactam group of antibiotics are the commonly prescribed antibiotics for this disorder. The emergence of antibiotic resistance among gramnegative bacilli limited applicability and usefulness of the antibiotics. In this study, we assessed the prevalence of extended spectrum beta-lactamases (ESBL) produced in E. coli, extracted from urine samples in Taleghani Hospital of Tehran. Material and Methods: This descriptive study was performed on patients with urinary tract infection, who referred to Taleghani Hospital, from April to July ۲۰۱۴. A total of ۱۸۹۶ patients with urinary tract infection were investigated. After gram staining, morphology, growth characteristics and biochemical tests of ۲۲۵ strains of E. coli were extracted from the samples. Following instructions of clinical laboratory standards institute (CLSI), diffusion disk method, sulfamethoxazoletrimethoprim disks, nitrofurantoin, tobramaysn, cefixime, cefpodoxime, piperacillin-tazobactam, ampicillin, cefazolin, ceftriaxone, amikacin, meropenem, colistin, ceftazidime, ciprofloxacin and gentamicin were used in Müller-Hinton agar medium to determine antibiotic sensitivity. Results: In this study, from the ۲۲۵ separated E. coli samples, belonged to males (۳۹.۲%) and ۱۳۷ were from females (۶۰.۸%). The mean age of the patients was ۴۱±۲۱ years. After determining ESBL-producing isolates through combined disk method, it was found that ۹۷ out of ۲۲۵ isolates were ESBL-producing. Conclusion: The rate of urinary tract infections caused by E. coli is still high. The investigations of resistance pattern demonstrate high level of E. coli resistance against various antibiotics, and it seems that this resistance is continuously increasing

کلیدواژه ها:

Beta-lactamase ، E. coli ، Urinary tract infection ، عفونت ادراری ، اشریشیا کلی ، بتالاکتاماز وسیع الطیف