Study on Immunity of an Experimental Oil Adjuvant Haemorrhagic Septicaemia Vaccine in Cattle.

An oil adjuvant vaccine (OAV) was prepared from a local strain of Pasteurella multocida. Strain ۶:B was cultured and inactivated by formalin. Bacterial pellet was prepared by centrifugation and subsequently adjuvanted by Montannide oil ISA-۷۰. A dose of prepared vaccine containing ۳ml (۲mg dry weight/ml) was injected into five calves by IM route. Animals were bled before and at ۲۴, ۹۰, ۱۵۰, and ۲۰۰ days post-vaccination. Collected sera were used in passive mouse protection test (PMPT). Active mouse protection test (AMPT) was carried out for OAV according to standard method. Results of PMPT showed ۱۰۰% protection up to ۱۵۰ days and ۶۶-۸۳% up to ۲۰۰ days post-vaccination. In AMPT, ۴ log of protection was gained. In this experiment the immunity induced by OAV adjuvanted by ISA-۷۰ could protect the calves.

کلیدواژه ها:

haemorrhagic septicaemia ، oil adjuvant vaccine ، Protection