PCR detection of thymidine kinase gen of latent herpes simplex Virus type ۱ in mice trigeminal ganglia
محل انتشار: مجله آرشیو رازی، دوره: 61، شماره: 3
سال انتشار: 1385
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 72
فایل این مقاله در 5 صفحه با فرمت PDF قابل دریافت می باشد
- صدور گواهی نمایه سازی
- من نویسنده این مقاله هستم
استخراج به نرم افزارهای پژوهشی:
شناسه ملی سند علمی:
JR_ARCHRAZI-61-3_002
تاریخ نمایه سازی: 6 دی 1402
چکیده مقاله:
Herpes simplex virus type ۱ establishes a latent infection in the peripheral nervous system following primary infection. During latent infection, virus genome exhibit limited transcription, with the HSV LATs consistently detected in latency infected ganaglia. Following ocular infection viral latency develops in the trigeminal ganglia. In this study PCR has been used for detection of HSV-۱ nucliec acid . BALB/c mice were inoculated with HSV-۱ and infected mice ganglia were applied to detect HSV-۱ TK gene. Amplification of TK fragment in mice ganglia was considered as an indicator of viral latency. The results were suggested that TK PCR can be used as a reliable diagnostic tool and it is a specific and highly sensitive method for the detection of HSV genome in trigeminal ganglia.
کلیدواژه ها: