Serological Evaluation of Experimental Toxoplasma gondii Infection in Cats by Using Immunoblotting Based on an Affinity Purified Surface Antigen
محل انتشار: مجله آرشیو رازی، دوره: 67، شماره: 2
سال انتشار: 1391
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 27
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شناسه ملی سند علمی:
JR_ARCHRAZI-67-2_010
تاریخ نمایه سازی: 6 دی 1402
چکیده مقاله:
Toxoplasma gondii is an apicomplexan parasite that infects human and almost all warm-blooded animals. The life cycle of the parasite includes an asexual reproduction in intermediate hosts (Mammals and birds) and a sexual reproduction in definitive hosts (Felidae). Cats are both the intermediate and the definitive host for T. gondii. The aim of this study was to investigate anti-T. gondii antibodies in cats by using an immunoblot method based on a major surface antigen; SAG۱. Six sero-negative kittens were infected intra-peritoneally by Rh strain of T. gondii. Serum samples were collected and evaluated for the presence of IgG antibodies by using two techniques; immunoblotting and indirect fluorescent antibody test (IFAT).SAG۱ based immunoblotting was able to detect anti-T. gondii antibodies at least eight days after infection. Comparative evaluation showed that this method is as sensitive as IFAT to diagnose T. gondii infection in cats.
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