Detection of Mycoplasma capricolum capricolum from goats of Qom province, Iran
محل انتشار: مجله آرشیو رازی، دوره: 70، شماره: 1
سال انتشار: 1394
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 71
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شناسه ملی سند علمی:
JR_ARCHRAZI-70-1_007
تاریخ نمایه سازی: 6 دی 1402
چکیده مقاله:
Mycoplasma capricolum subsp capricolum (Mcc) is one of the etiological agents of contagious agalactia(C.A) in goats which can cause significant economic losses. The aim of this study was to detect Mcc from goats of Qom province in Iran. A total of ۱۱۱ samples were collected from suspected goats to C.A and cultured in PPLO broth supplemented for Mcc isolation. The bacteria DNAs were extracted from clinical samples and the PCR assay was performed to detect Mycoplasma genus, cluster Mycoides and Mcc from culture. Out of the ۱۱۱ samples, ۳۳(۲۹.۷%) sample cultures were shown positive and typical Mycoplasma colonies in PPLO agar culture method, ۵۳ (۴۷.۷%) samples were scored positive by Mycoplasma genus PCR, ۸ (۷%) of the samples were scored positive by using myciodes cluster PCR and finally ۲(۱.۸%) of the samples were scored positive by using Mcc PCR method. The result of this study showed that Mcc was detected for the first time from Qom province in Iran. Therefore, Qom could be one of the geographical distribution and efficient factors of Mcc in Iranian goats. The lung samples and lymph nodes also could be significant samples for detection of Mcc.
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