Changes in some pro-and anti-inflammatory cytokines produced by bovine peripheral blood mononuclear cells following foot and mouth disease vaccination
محل انتشار: مجله آرشیو رازی، دوره: 71، شماره: 3
سال انتشار: 1395
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 85
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شناسه ملی سند علمی:
JR_ARCHRAZI-71-3_008
تاریخ نمایه سازی: 6 دی 1402
چکیده مقاله:
Interleukin (IL)-۱۷ is exclusively produced by CD۴ helper T-cells upon activation. It most often acts as a pro-inflammatory cytokine, which stimulates the release of pro-inflammatory cytokines IL-۶, IL-۸, TNF-α, and granulocyte-macrophage colony-stimulating factor (GM-CSF). In this study, we studied the in-vitro IL-۱۷ response to specific antigens and a variety of mitogens and compared the IL-۱۷ response to IL-۲, IL-۴, IL-۵, IL-۶, IL-۱۰, and IFN-γ responses. We used a foot and mouth disease (FMD) vaccine as specific antigens and mitogens (phytohemagglutinin [PHA], pokeweed mitogen [PWM], and concanavalin A [Con A]) to stimulate peripheral blood mononuclear cells (PBMCs) of vaccinated calves. Cell culture supernatant was harvested and analyzed for cytokines, using commercially available bovine ELISA kits. The mitogens induced a significant increase in IL-۱۷ production. IL-۱۷ was produced at high levels in response to the T cell-stimulated mitogens, PHA, and Con A, and at low levels in response to PWM mitogens. In contrast, level of the produced IL-۱۷ cytokines in response to the FMDV antigens was lower as compared to those produced by mitogens. The FMDV antigens and mitogens significantly increased IL-۱۷ production. There was not a correlation between IL-۱۷ production and type-۱ cytokine, IFN-γ, and IL-۲, while there was a correlation between type-۲ cytokine, IL-۴, and IL-۵ at either cytokine level produced by PBMCs stimulated by FMDV antigens. Moreover, there was an interaction between IL-۱۷ and IL-۶, that is, as IL-۶ cytokine level elevated or diminished, IL-۱۷ cytokine level increased or decreased, as well.
کلیدواژه ها:
نویسندگان
N. Delirezh
Department of Microbiology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
R. Norian
Department of Microbiology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
A. Azadmehr
Department of Immunology, Qazvin University of Medical Sciences, Qazvin, Iran
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