Diagnosis of Avian Mycoplasmas: A Comparison between PCR and Culture Technique

Mycoplasma gallisepticum and Mycoplasma synoviae are the causative agents of avian mycoplasmosis in commercial poultry. Among the available tools, polymerase chain reaction (PCR) and culture are confirmatory tools for the diagnosis of mycoplasmosis after the initial serological screening of suspected birds. Overall, ۱۸۱ samples were analyzed, ۱۵۲ (۸۴%) and ۱۰۳ (۵۷%) of which were found positive by PCR and culture, respectively. Further, ۵۴ (۹۲%) broiler samples were found positive for general avian mycoplasma. Among the total positive samples, MS positivity was as high as ۷۲ (۴۷%) by PCR, while it was ۴۵ (۴۴%) by culture. MG positivity was ۲۳% and ۲۵% in PCR- and culture-positive samples. MG grows more easily compared to MS. The agreement value between the tests was ۶۷%. Overall, flock wise prevalence was not much varied. The prevalence of mycoplasmosis was higher during winter. Our study confirmed that PCR is the most sensitive and reliable tool for the diagnosis of avian mycoplasmosis in field samples.

کلیدواژه ها:

Avian Mycoplasmosis ، Mycoplasma gallispticum ، Mycoplasma synoviae ، PCR ، Culture