Determination of the Effective Dose of Curcumin alone and in Combination with Antimicrobial Peptide CM۱۱ on Promastigote Forms of Iranian Strain of L. major (MRHO / IR / ۷۵ / ER)

سال انتشار: 1398
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 70

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شناسه ملی سند علمی:

JR_ARCHRAZI-74-4_009

تاریخ نمایه سازی: 6 دی 1402

چکیده مقاله:

Zoonotic cutaneous leishmaniasis t caused by Leishmania major is spread in focal areas of more than ۹۰ countries in the tropics, subtropics, and southern Europe. In the absence of any effective vaccine, the only means to treat and control leishmaniasis is conventional medication. Glucantime is the first choice of anti-leishmanialdrug, has serious side effects like high toxicity, exorbitant cost, problems with the administration and development of resistance. Curcumin is the active component from the rhizome of herb Curcuma longa, possessing many pharmacological and biological activities with antiprotozoal and anti-proliferative effects which make it a good alternative to existing therapy. Antimicrobial peptides like CM۱۱, a small peptide consisting of ۱۱ amino acids, are also novel potential drugs against at least wide spectrum of microbial organisms. The aim of this study was to evaluate the effect of curcumin alone and in combination with CM۱۱ on promastigote form of L. major (MRHO / IR / ۷۵ / ER) for ۱۲h and ۲۴h in vitro. The results of Giemsa staining showed that the morphology of the flagellum and cell shape increased changed with increasing concentration of curcumin (۵ µM, ۱۰ μM, ۲۰ μM, ۴۰ μM and ۸۰ μM). MTT and Trypan blue results demonstrated that the promastigotes were susceptible against curcumin in dose and time dependent manner, while CM۱۱ alone at concentration of ۸ µM as well as in combination with ۱۰ and ۲۰ µM curcumin had no significant effect on promastigotes. Our results revealed that curcumin can provide a new curative candidate against cutaneous leishmaniasis.

نویسندگان

G. Aqeele

Department of Parasitology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran

P. Shayan

Department of Parasitology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran

E. Ebrahimzadeh

Department of Parasitology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran

M. Mohebali

Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran

S. Khalili

Department of Parasitology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran

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