Effect of Carum copticum nano-essence against Saprolegnia and Fusarium, and the Use of Multiplex PCR Assay for the Detection of These Organisms in Rainbow trout Oncorhynchus mykiss
محل انتشار: مجله آرشیو رازی، دوره: 76، شماره: 2
سال انتشار: 1400
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 56
فایل این مقاله در 11 صفحه با فرمت PDF قابل دریافت می باشد
- صدور گواهی نمایه سازی
- من نویسنده این مقاله هستم
استخراج به نرم افزارهای پژوهشی:
شناسه ملی سند علمی:
JR_ARCHRAZI-76-2_006
تاریخ نمایه سازی: 6 دی 1402
چکیده مقاله:
This study aimed to investigate the fungal species isolation and confirmation by the Multiplex PCR method in aquatic fish. Evaluation of the inhibitory effect of nano-essential oils of Carum copticum on isolated fungal species was also conducted in this study. The PCR results showed that ۳ out of ۵ samples were diagnosed with Fusarium solani, and two of them were positive for Saprolegnia. Moreover, in ۰.۱% of the females' nanoparticles, one peak appeared that showed a particle with an average diameter of ۳۶۰ nm, and two nanoparticles showed a peak with a mean diameter of ۲۴۲ nm. The results of minimum inhibitory concentrations (MIC) and minimum fungicidal concentrations (MFC) showed that ۰.۰۱% nano essential oil had ۰.۰۸ and ۰.۰۷ mg/ml MIC values against Fusarium solani and Saprolegnia, respectively. Gram/ml was on the growth of Fusarium solani species. The essential oils of female plants had an MIC of ۰.۰۷ in ۰.۱% essential oil and ۰.۰۳ mg/ml in ۰.۰۱% essential oil in Saprolegnia. Furthermore, in the case of ۰.۱% nano essential oil, the results showed the MIC values of ۰.۰۴ and ۰.۰۳ mg/ml against Fusarium solani and Saprolegnia, respectively. The MFC values of ۰.۱% nano essential oil were ۰.۱ and ۰.۰۷ mg/ml against Fusarium solani and Saprolegnia,respectively. It was not found on Fusarium and Saprolegnia. Overall, the results of this study using PCR for direct detection showed that ۷۰% and ۵۰% of the samples were Fusarium solani and Saprolegnia positive, respectively; therefore, the PCR was an efficient method for the detection of fungi. According to the results of nano-essential oil (۰.۱%) of females, this nano-essence had a strong inhibitory effect on Fusarium solani and Saprolegnia.
کلیدواژه ها:
نویسندگان
P Tour SavadKouhi
Department of Food Sciences and Technology, Science and Research Branch, Islamic Azad University, Tehran, Iran
H Ahari
Department of Food Sciences and Technology, Science and Research Branch, Islamic Azad University, Tehran, Iran
A. A Anvar
Department of Food Hygiene, Science and Research Branch, Islamic Azad University, Tehran, Iran
B Jafari
Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran
مراجع و منابع این مقاله:
لیست زیر مراجع و منابع استفاده شده در این مقاله را نمایش می دهد. این مراجع به صورت کاملا ماشینی و بر اساس هوش مصنوعی استخراج شده اند و لذا ممکن است دارای اشکالاتی باشند که به مرور زمان دقت استخراج این محتوا افزایش می یابد. مراجعی که مقالات مربوط به آنها در سیویلیکا نمایه شده و پیدا شده اند، به خود مقاله لینک شده اند :