Study of Some Resistance Genes in Clinical Proteus mirabilis

Proteus mirabilis belongs to the family Enterobacteriaceae and is capable of transforming in shape from rod to elongated and swarming motility by flagella. It is an opportunity for bacteria and can cause different clinical diseases. Therefore, this study aimed to assay and detect a sequence of genes that encode for antibiotic resistance in multidrug resistance clinical isolates of Proteus mirabilis, including blaTEM, aac(۶’)-Ib, qnrA, IntI۲, IntI۱ and secondly to investigate the relationship in the phylogenetic tree among these genes in Iraq comparison with global strains in NCBI. The study included the identifying of ۵۰۰ clinical samples depending on morphological and biochemical tests and confirming Proteus mirabilis diagnosis by the VITEK-۲ Compact system. The confirmed isolates of Proteus mirabilis were ۹۵ clinical isolates (۱۹%). Antibiotic susceptibility test of all these isolates was done using twelve antibiotics tested using Amoxicillin, Aztreonam, Imipenem, Cefoxitin, Amikacin, Ceftazidem, Ciprofloxacin, Nalidixic acid, Gentamicin, Sulphamethazol-trimethoprim, Cefotaxime, Amoxicillin-clavulanic acid. The results showed that multidrug resistance Proteus mirabilis isolates contained the genes in different levels as follow blaTEM gene (۹۰%), aac(۶’)-Ib  gene (۸۰%) ,IntI۱ gene (۱۰۰%), IntI۲ gene (۸۰%). These genes were sequenced and detected phylogenetic relationships among these genes and global genes were documented in NCBI. The results showed that some Iraqi isolates contain genetic variation compared to global strains. Therefore, this variation was detected and registered in NCBI of all five antibiotic resistance genes mentioned above and accepted under accession numbers of aacIb gene (LC۶۱۳۱۶۸.۱), blaTEM gene (LC۶۱۳۱۶۶.۱), IntI۱ gene (LC۶۱۳۱۶۹.۱), IntI۲ gene (LC۶۱۳۱۷۰.۱).

کلیدواژه ها:

Multidrug Proteus mirabilis ، Antibiotic resistance genes ، Integrons ، Iraqi clinical samples ، NCBI