L-Theanine Improves Locomotor Function in a Model of Multiple Sclerosis Mice

This study designed to investigate the protective effects of L-theanine on experimental Multiple sclerosis in mice. Frothy Male C۵۷BL/۶ mice were allocated into ۴ experimental groups: control no treatment received a regular chew pellet, and the cuprizone (CPZ) group received a standard chew pellet containing ۰.۲% (w/w) CPZ. In group ۳, mice were fed a regular diet and administered p.o. with L-theanine (۵۰ mg/kg). In group ۴, mice received a diet containing CPZ and were administered p.o. with L-theanine (۵۰mg/kg). Finally, reflexive motor behavior and serum antioxidant levels were determined. Based on findings, CPZ significantly decreased ambulation score, hind-limb suspension, front limb suspension, and grip strength (P<۰.۰۵). The CPZ+L-theanine reduced the adverse effect of the CPZ on ambulation score, hind-limb foot angle, surface righting, and negative geotaxis (P<۰.۰۵). The CPZ+L-theanine increased front and hind-limb suspension, grip strength, number of the cross, and duration of a stay on the rotarod compared to the control animal (P<۰.۰۵). CPZ administration significantly elevated serum malondialdehyde (MDA) while superoxide dismutase (SOD) and glutathione peroxidase (GPx) and total antioxidant status (TAS) levels decreased compared to control mice (P<۰.۰۵). The CPZ+L-theanine leads to the cessation of MDA production while increasing SOD, GPx, and TAS levels (P<۰.۰۵). These results suggested L-theanine has a protective effect against CPZ-induced MS in mice.

کلیدواژه ها:

Antioxidant ، L-theanine ، Multiple sclerosis ، Mice