Effects of the Melatonin and Vitamin E (Alpha-Tocopherol Acetate) as Antioxidants on Biochemical Blood Parameters, Lipid Profile, and Muscle Vitamin E Concentration in Awassi Lambs Fed a High-Energy Diet and Normal Diet

Health specialists currently suggest low-cholesterol diets, suggesting that cholesterol in the form of high-density lipoprotein (HDL) reduces the risk of chronic atherosclerosis. The large volume of literature describes the biological roles of vitamin E and its application to preventing disease and improving the health and productive performances of farm animals. The present study aimed to evaluate the effects of vitamin E (Alpha-tocopherol acetate) supplementation and melatonin implants on biochemical blood, lipid profile and muscle vitamin E content of Awassi male lambs fed by a high and normal diet in Iraq. The lambs were divided into teen groups as control normal energy diet T۱ (NED) T۲ (HED) concentrated lamb fattening feed. Two levels of melatonin (۱۸ and ۳۶ mg implant) were applied to T۳, T۴, T۵, and T۶ treatment and ۲ levels of Vitamin E (Alpha-tocopherol acetate) diet ۲۰۰ mg/kg, ۴۰۰ mg/kg to T۷. T۸. T۹ and T۱۰, respectively. Results from the present study indicate that Vitamin E ۲۰۰, ۴۰۰ mg/lamb/day and melatonin implantation ۱۸ mg, ۳۶ mg/lamb/day significantly (P<۰.۰۵) increased total protein in serum while decreasing globulin level, glucose concentration in serum, melatonin implantation ۳۶ mg/lamb and vitamin E ۴۰۰ mg/lamb/day recorded significantly (P<۰.۰۵). The same effect on decreasing cholesterol concentration in serum ۴۲.۶mg\dl, ۴۰.۵ mg\dl, respectively, compared to non-treated groups. Vitamin E ۲۰۰ mg/kg/lamb recorded the lowest AST level in serum, ۴۳.۳. Lambs implanted with Melatonin ۳۶ mg/lamb and fed a high-energy diet (T۸) resulted in a significant decrease of serum ALT activity (P<۰.۰۵) in comparison to other treated groups ۱۲.۷ U/L was achieved. Lambs fed a normal energy diet with vitamin E ۲۰۰ mg/kg/lamb (T۴) exceeded other treated groups, decreasing ALT serum levels by ۹.۳۵ U/L. Interestingly, muscle vitamin E concentrations for lambs received ۲۰۰, ۴۰۰ mg/lamb/day on the ۲nd, ۷th and ۱۴th days of the storage period, and fed high energy diet (T۱۰) or normal energy diet (T۵) were significantly higher compared to control group (T۱, T۶).

کلیدواژه ها:

Awassi lamb ، Melatonin implants ، Alpha-tocopherol acetate ، blood biochemical trait ، LD muscle vitamin E concentration ، lipid profile