Isolation of Pseudomonas aeruginosa from Persistent Bacterial Coinfection of a COVID-۱۹ Patients with Molecular Detection of Antibiotics Resistance Genes

Pseudomonas aeruginosa (P. aeruginosa) have a considerable risk to public health in the world, due to its high ability to develop resistance to different classes of antibiotics. It has been discovered as a prevalent coinfection pathogen that causes sickness exacerbation in COVID-۱۹ patients. This study aimed to determine the prevalence of P. aeruginosa from COVID-۱۹ patients in Al Diwaniyah province, Iraq and to identify its genetic resistance pattern. ۷۰ clinical samples were obtained from severe cases of patients (RT-PCR positive for SARS-COV-۲ on a nasopharyngeal swab) who attended Al Diwaniyah Academic Hospital. ۵۰ P. aeruginosa bacterial isolates were detected via microscopic examination, routine cultured and biochemical testing, then validated by the VITEK-۲ compact system. VITEK reported ۳۰ positive results, which later confirmed through molecular detection using ۱۶s RNA specific for detection and a phylogenetic tree.۲۰ isolates had positive PCR findings and ۵ isolates submitted to GenBank with accession numbers OL۳۱۴۵۵۷.۱, OL۳۱۴۵۵۶.۱, OL۳۱۴۵۵۵.۱, OL۳۱۴۵۵۴.۱, OL۳۱۴۵۵۳.۱.For antibiotic resistance genes, the number of the isolates containing blaOXA-۱ and blaCTX-M ۱۸ (۹۰ percent) and ۱۶ (۸۰ percent) respectively. To study its adaptation in a SARS-CoV-۲ infected environment, genomic sequencing investigations were undertaken with phenotypic validation. In conclusion, we demonstrate that multidrug resistant P. aeruginosa play an important role in in vivo colonization in COVID-۱۹ patients and could be one of the causes of death of these patients which indicates the great challenge to clinicians in the facing of this serious disease.

کلیدواژه ها:

covid-۱۹ ، Pseudomonas aeruginosa ، bla OXA-۱ ، bla CTX-M ، Antibiotic resistance ، coinfection