Diagnosis of Oral candidiasis in Patients under ۱۲ Years: ۱۸S rRNA as a Marker of Molecular Characterization of Candida tropicalis

Candida tropical has been found as the most abundant pathogenic yeast species under the group Candida-non-albicans. Despite this, it is taxonomically related to C. albicans and has many of its pathogenic characteristics. Infection with Candida tropicalis is closely associated with many virulence factors encoded by multiple virulence genes. This study aims to diagnose C. tropicalis based on the presence of ۱۸SrRNA and to detect many virulence genes. C. tropicalis isolates were collected from oral candidiasis patients. Children infected with oral thrush ranging in age from infants to ۱۲ years old provided ۱۵۰ samples. C. albicans (۶۶.۶۸ %), C. tropicalis (۱۳.۲۱ %), C. krusie (۹.۴۳ %), C. parapsilosis (۷.۵۵ %), and C. glarata were isolated as C. tropicalis types, according to the findings of the present study (۲.۸۳%). The presence of the ۱۸SrRNA gene was confirmed in the isolates. All isolates were positive for cph۱ and hwp۱, while some were positive for sap۱ (۷۸.۵%) and plb۱ genes (۷۱.۴%). Using sequences and phylogenetic trees, it was determined that there was negligible genetic variation between local isolates and global strains. These virulence factor genes play a crucial role in developing infections.

کلیدواژه ها:

Oral candidiasis ، sap۱ ، hwp۱ ، cph۱ ، plb۱