The Healing Effect of Biodegradable Scaffolds Treated with Bone-Marrow Obtained Mesenchymal Stem Cells on Major Tendon Damage in the Dog as a Model

The present study aimed to evaluate the implantation of decellularized small intestinal submucosa- extracellular matrix )SIS-ECM( seeded with bone marrow mesenchymal stem cells (BM-MSCs) to repair full-thickness Achilles tendon defect. For this purpose, ۲۰ healthy adult stray dogs aged ۸-۱۲ months old (۱۵±۳ kg of weight) were enrolled in this study under an aseptic environment and general anesthesia. A ۱.۵ cm-long segment-based resection was performed in the mid-substance of the Achilles tendon in the control group (n=۱۰) that did not receive treatment. While, in the experimental group (n=۱۰), regarding the defect of the tendon, the stumps were bridged with decellularized SIS seeded with BM-MSCs (۵×۱۰۶) cells implanted. Afterward, the stumps of the tendon were sutured using the modified Kessler technique (۴-۰) polypropylene thread. The biomechanical observations of the tendon defect showed an increase in the tensile strength in the experimental group, compared to the control animals. It should be mentioned that this difference was significant (P≤۰.۰۵). Histopathological observations of biopsies harvested after the ۴th, ۸th, and ۱۲th weeks revealed that the implanted graft had seeded with MSCs enhanced high-quality cellular infiltration and the host tissue healing was improved. Similar to the normal tendon, a dense organized collagenous tissue with high cellularity and vascularity was observed due to the presence of the remodeled ECM. However, the arrangement of collagen-fiber-derived connective tissue appeared to be more dominant than that in the experimental group, with less adhesion in the ۱۲th week post-treatment. These findings suggest that the BM-MSCs inoculated with SIS can be employed to repair a damaged Achilles tendon due to the fact that this combination enhances the regeneration of the affected tendon.

کلیدواژه ها:

Achilles tendon ، Bone marrow ، Dog ، Implantation ، Mesenchymal stem cells-Small intestinal submucosa (SIS) ، Tendon-healing