The Impact of Berberine Loaded Selenium Nanoparticles on K. pneumoniae and Candida albicans Antibiotics Resistance Isolates

This study aimed to investigate the antibacterial and antifungal activities of selenium nanoparticles (SeNPs) and berberine (BBR) despite antibiotic resistance against Klebsiella pneumoniae and Candida albicans. Cells of K. pneumoniae and C. albicans were treated with solutions of different concentrations of each bare SeNPs, BBR, and BBR-loaded SeNPs (BLS) using the disk diffusion method. The results indicated that the activities of SeNPs, BBR, and BLS were statistically significant (P<۰.۰۵) when the concentration of all agents increased. Moreover, it was found that BLS had a statistically significant effect against K. pneumoniae and C. albicans, compared to SeNPs and BBR alone (P<۰.۰۵). The largest zones of inhibition of SeNPs were ۱۴ and ۱۶ mm toward K. pneumoniae and C. albicans, respectively, at the concentration of ۲۰ Mml, compared to the concentrations of ۱۰ and ۱۵ Mml. Furthermore, BBR showed a maximum zone of inhibition at the concentration of ۱,۲۰۰ mg (۱۵ mm for K. pneumoniae and ۱۸ mm for C. albicans) and it was statistically significant in comparison with other concentrations of ۴۰۰ and ۸۰۰ mg. In addition, the BLS underwent a statistically significant increase (P<۰.۰۵) when the concentration increased and it registered a large zone of inhibition of ۲۲ and ۲۵ mm against K. pneumoniae and C. albicans, respectively, at ۲۰ Mml of SeNPs: ۱,۲۰۰ mg BBR, compared to ۱۰ Mml of SeNPs: ۴۰۰ mg BBR and ۱۵ Mml of SeNPs: ۸۰۰ mg BBR. Based on the results of the current study, there was a statistically synergistic effect of BBR-loaded SeNPs, compared to that of BBR and Se nanoparticles, only in the case of both K. pneumoniae and C. albicans. This study is promising as a blueprint for the enhancement of weak antimicrobial agents and their return to their previous role as antibiotics.

کلیدواژه ها:

Berberine ، Candida albicans ، K. pneumoniae ، Selenium nanoparticles