Effects of β-Hydroxy-β-Methylbutyrate Supplementation on IL-۴, IL-۱۰ and TGF-β۱ during Resistance Exercise in Athletes

The aim of this study was to investigate the effect of β-Hydroxy-β-methylbutyrate (HMB) supplementation on anti-inflammatory cytokines including IL-۴, IL-۱۰ and TGF-β during an acute bout of resistance exercise (RE) in young resistance trained men. Ten resistance-trained men in a randomized, double-blind, placebo-controlled and crossover study, were administered a ۷-day HMB supplementation (۳×۱ g.d-۱ of HMB) and placebo (۳×۱ g.d-۱ of Maltodextrin) with a ۷ days washout period. After supplementation periods, subjects performed three sets of bench press, lat pull down, leg extension, leg curl, biceps curl, triceps curl and shoulder press to failure with ۸۵% of one repetition to maximum (۱RM). Blood samples were obtained before- (Pre), immediately post- (IP) and ۱ hour-post RE (۱h P) to assess serum concentrations of IL-۴, IL-۱۰ and TGF-β۱. The data were analyzed using ۲ (treatment: HMB and PL) × ۳ (time points: Pre, IP and ۱hP) repeated measures analysis of variance (ANOVA) followed by the Bonferroni post hoc test with a significant level of p<۰.۰۵. Serum IL-۴ was significantly higher at IP resistance exercise in HMB compared to placebo. Circulating IL-۴ and TGF-β۱ were significantly raised at IP compared to Pre in both HMB and placebo treatments. No significant differences between treatments were observed for IL-۱۰ and TGF-β۱at any time points. In conclusion, HMB supplementation increased the circulating level of IL-۴ during RE in resistance-trained men, which may attenuate inflammation and facilitate adaptation to RE.

کلیدواژه ها:

β-Hydroxy-β-Methylbutyrate ، anti-inflamatory cytokines ، resistance exercise