Therapeutic effects of Medicago sativa against cyclophosphamide-induced toxicity in rats
محل انتشار: مجله گیاهان دارویی ابن سینا، دوره: 14، شماره: 1
سال انتشار: 1403
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 52
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شناسه ملی سند علمی:
JR_AJP-14-1_009
تاریخ نمایه سازی: 23 آذر 1402
چکیده مقاله:
Objective: Medicago sativa (M. sativa) has been traditionally used for treating anemia; therefore, M. sativa hydro-ethanolic extract therapeutic effects against cyclophosphamide (CP) -induced hematologic and liver toxicity were examined. Materials and Methods: Thirty male Wistar rats were randomly divided to control (saline); CP (۱۰۰ mg/kg, day ۱-۳, subcutaneously); CP+ M. sativa ۲۰۰ mg/kg (MS ۲۰۰); CP+ M. sativa ۴۰۰ mg/kg (MS ۴۰۰); CP+ dexamethasone (۰.۱ mg/kg), (all groups n=۶). Treated animals received M. sativa or dexamethasone by gavage from days ۷-۱۴. On days ۰, ۷, and ۱۴, hematologic parameters, and on the ۱۴th day, serum and liver tissue oxidative stress markers including nitric oxide, malondialdehyde (MDA) and total thiol levels, superoxide dismutase (SOD) and catalase (CAT) activities, serum lipids, and liver enzymes were measured.Results: Animal weight, platelet, white blood cells, and red blood cells counts, hemoglobin and hematocrit as well as thiol, SOD, and CAT activities in serum and liver tissue were significantly reduced, but serum nitric oxide, MDA, total cholesterol, triglycerides, low-density lipoproteins levels, and liver enzymes were increased in the CP group compared to the control group (p<۰.۰۵ to p<۰.۰۰۱). Administering M. sativa extract (۴۰۰ mg/kg) significantly enhanced platelet count, and SOD and CAT activities and inhibited all of the CP toxic effects, while dexamethasone improved platelet count and oxidative stress markers compared to the CP group (p<۰.۰۵ to p<۰.۰۰۱).Conclusion: The extract of M. sativa (۴۰۰ mg/kg) showed therapeutic effects against the CP-induced myelosuppression and thrombocytopenia and improved oxidative stress markers which were comparable to the effect of dexamethasone.
کلیدواژه ها:
نویسندگان
Vajihe Rouki
Department of Physiology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
Mohammad Hossein Boskabady
Department of Physiology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
Narges Marefati
Department of Physiology and Medical Physics, Faculty of Medicine, Baqiyatallah University of Medical Sciences, Tehran, Iran
Reyhaneh Sotoudeh
Department of Physiology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
Zahra Gholamnezhad
Department of Physiology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran