CCL۲۰ and CNTNAP۳C as prognostic biomarkers and therapeutic targets in HCT۱۱۶ cell lines treated with Fusobacterium nucleatum for colorectal cancer metastasis

Colorectal cancer (CRC) is the fourth most commonly diagnosed cancer worldwide and the second most frequent cause of death closely following lung cancer. Cancer metastasis, a major clinical problem of human cancer, is responsible for most colorectal cancer patient mortality. Thus, it is important to elucidate the underlying mechanisms of metastasis in CRC patients. F. nucleatum is a Gram negative anaerobic oncogenic bacterium enriched in human colorectal carcinoma compared with adjacent normal tissues. However, the potential e↵ects and underlying mechanisms of F. nucleatum in the interactions between cells in CRC metastasis remain largely unknown. Material and Method:SRP۳۱۳۷۸۰ dataset was downloaded from https://www.ncbi.nlm.nih.gov/sra , the sra file was converted to two Fastq paired files with sratoolkit.۲.۱۱.۳- software. Quality control of Fastq files was performed with FASTQC software, and low quality reads were trimmed with Trimmomatic software and we mapped the trimmed file to GRCH۳۸ genome using Hisat۲ software and sam file was obtained. In the next step, we converted the sam file to count file using htseq-count software and finally, DEGs were identified using the DESeq۲ package in R Studio software by threshold (FDR:۰.۰۵ and log۲foldchange>۱).Results:Based on the results, in the HCT۱۱۶ cell lines treated with Fusobacterium nucleatum compared with control group, upregulated genes included: CCL۲۰, CXCL۸, CSF۲, CXCL۱ and CXCL۲. Downregulated genes included: CNTNAP۳C, NCR۳LG۱, LOC۱۰۰۱۲۸۶۵۳, EGFR-AS۱ and ANKRD۳۶BP۱.Conclusion:The overabundance of F. nucleatum in CRC tissues has been reported to promote CRC cell proliferation, recurrence and chemo resistance. Due to the important role of Fusobacterium nucleatum in cell proliferation, differentiation, apoptosis and signaling pathways, genes that up and down expression, were used for prevention, diagnosis, treatment and reduction of metastasis in patients with colorectal cancer.

کلیدواژه ها:

Colorectal Cancer ، Fusobacterium nucleatum ، prognostic biomarkers ، therapeutic targets