Investigating the pathway of resistance to Ciprofloxacin inCampylobacter jejuni by using microarray analysis

C. jejuni is one of the main and e↵ective Gram-negative bacteria in food poisoning and causing gastrointestinal infections in the intestine. This bacterium is a hidden microbe in food, so-called foodborne, which is able to cause infection by penetrating the small intestine, and for this reason, it has become one of the main sources of digestive and internal infections. The main challenge in dealing with C. jejuni infections is the resistance of strains of this species to various antibiotics (Iovine,۲۰۱۳). Ciprofloxacin resistance is common among animal and human C. jejuni isolates . Investigating gene expression profiles in antibiotic resistant strains of this disease can clarify the process of resistance and pathogenicity of resistant strains and play a role in designing new drugs, prescribing antibiotics and finally eradicating C. jejuni infections be effective. Data were loaded into the R software environment after being downloaded from the NCBI GEO database with the accession ID GSE۴۱۸۲۲. In order to normalize the data, we used the DESeq۲ library. Then, with the help of examining differential gene expression by means of DESeq۲ library and using statistical tools such as effect function, dispersion and curve fitting, genes with differential expression between different groups were identified, and the significant difference in the expression of each among the groups was determined a p-value was given. Hierarchical clustering (hClust) was used to investigate the difference in gene expression. We found that in general, M۲۳/M۳۷ protease and cytochrome genes and genes involved in the transfer of substances such as transferases can be considered the most common genes in Ciprofloxacin antibiotic and these genes will be possible candidates for inhibitor drugs

کلیدواژه ها:

Campylobacter jejuni ، antibiotic resistance ، Ciprofloxacin ، Microarray analysis