Integrated bioinformatics analysis revealed that hub genes correlated with the prognosis and pathogenesis of gastric cancer

Gastric cancer is a major health issue in many countries, with a high prevalence in Asia, Africa, South America and Eastern Europe. Helicobacter pylori is associated with the development of several lesions in the human stomach. This chronic infection produces gastritis, which can progress to intestinal metaplasia and gastric cancer. Nextgeneration sequencing technologies have become widely used and cancer genomic analysis has recently revealed the relationships between various malignant tumors and genomic information. We aimed to elucidate potential candidate hub genes and key pathways related to gastric cancer based on bioinformatics analysis. GSE ۱۱۶۳۱۲ microarray dataset including gastric cancer biopsy and follicular gastritis biopsy were selected from GEO gene expression database and analyzed using GEO۲R online tool. The di↵erentially expressed genes (DEGs) between gastric cancer biopsy and follicular gastritis biopsy were analyzed by utilizing the R software. The shared DEGs were analyzed by Gene Ontology (GO) and functional enrichment. Protein-protein interactions (PPI) were constructed by utilizing the STRING database. Hub genes were analyzed by MCODE and Cytohubba. A total of ۸۵۲ shared DEGs between gastric cancer biopsy and follicular gastritis biopsy samples were identified. Gene function and KEGG pathway enrichment revealed that DEGs were mainly enriched in focal adhesion, gastric acid secretion, protein digestion and absorption and metabolic pathway. Eight modules were clustered based on PPI network analysis. The three main modules with the highest score were identified with a score of ۱۱.۵۲, ۷.۴۲ and ۷, respectively. Ten hub genes were identified by PPI network analysis respectively include: FN۱, IL۶, MYC, COL۱A۱, CXCL۸, SOX۲, THY۱, THBS۱, SPP۱ and CTGF. Taken together, the identification of these ۱۰ hub genes and enrichment pathways might have important clinical implications for gastric cancer treatment and diagnosis. The hub genes in this research can be considered as potential and valuable prognostic and pathogenesis biomarkers in gastric cancer.

کلیدواژه ها:

Helicobacter pylori ، Gene expression ، Gastric cancer ، Hub genes ، Bioinformatic analysis ، Microarray data