Bioinformatic Investigation Of Protein Stability (SQS) In Prokaryotes and Eukaryotes

Squalane is a triterpene hydrocarbon found in olive, palm, sa✏ower and rice bran oils. Squalane is an unsaturated triterpene that has wide applications in pharmaceuticals. In this research, squalene production and bioinformatic analysis of its gene in dracocephalum kotschyi plant as eukaryote and in Stigmatella aurantiaca bacterium as prokaryote were investigated in order to investigate the di↵erence of this gene in eukaryote and prokaryote. Among the medicinal uses of squalene, we can mention its use as an adjuvant of vaccines, inhibitor of cancer cells and antioxidant property. GC percentage of SQS protein was evaluated by GC Content Calculator, as well as aliphatic index and instability index by Protparam. The results of the study showed that the percentage of GC was ۶۰% in the studied eukaryote and ۶۳% in the studied prokaryote, which is close to each other in both cases. The higher the GC percentage of the examined protein, the more stable that protein is. Also, the instability index and aliphatic index for SQS- breeding in the studied eukaryote were higher than ۴۰ (۵۴/۲۴) and less than ۱۰۰ (۸۴/۹۴), respectively, and the studied prokaryote was ۴۸.۴۲ and ۱۵.۶۲, respectively. The instability index in the two investigated groups is close to each other, but the aliphatic index in prokaryotes is much lower than in eukaryotes. The instability index in the two investigated groups is close to each other, but the aliphatic index in prokaryotes is much lower than in eukaryotes. But the results of the investigation showed that both in prokaryotes and in eukaryotes, SQS protein is one of the semi-stable proteins

کلیدواژه ها:

GC Percentage ، Isoelectric ، Aliphatic Chains ، Relative Protein Volume ، SQS Protein