Reconstruction of miRNA-mRNA regulatory network in the pediatric Adrenocortical Adenoma

Pediatric Adrenocortical Tumors (ACT) are a rare disease in children, and Adrenocortical Adenoma (ACA) is one of its benign forms . MicroRNAs (miRNAs) are small noncoding RNAs that have been shown to be potential biomarkers for a variety of diseases, including cancer . In this study, we aim to identify diagnostic biomarkers of pediatric ACA by transcriptome data analysis. First, based on the Gene Expression Omnibus database, gene expression profiles related to ACA were downloaded. Di↵erentially expressed genes (DEGs) were analyzed with GEO۲R tool. Then, a defined formula [-log(p.value) × | log (fold change) | ] was applied to select the ۱۰۰۰-top significant probs. Protein-protein interaction (PPI) network was established through the STRING database. As well as using the Toppgene database for Gene Ontology (GO), we used it to select target miRNAs related to ACA hub genes. Next, the miRNA-mRNA regulatory network is reconstructed via Cytoscape software. After drawing the network, hub mRNA (FOS, SIRT۱, ESR۱, COL۱A۲, THBS۱) and miRNA (miR-۲۶a, miR-۲۹c, miR-۲۹b, miR-۲۹a, let-۷b) with the highest degree were determined. The most frequent top signaling pathway were IL-۴, ۱۳ and IL-۱۸ signaling pathway. We reconstructed a miRNA-mRNA network that may show crucial functions in the ACA, thus providing potential diagnostic biomarkers and therapeutic targets for ACA.

کلیدواژه ها:

biomarkers ، miRNA ، Adrenocortical Adenoma