Comparison of the Scolicidal Activity of Pulicaria gnaphalodes and Alhagi maurorum Extracts against Protoscoleces of Echinococcus granulosus Sensu Lato In Vitro

Background: Cystic echinococcosis (CE), caused by the larval stage of the Echinococcus granulosus, is a zoonotic disease and has a global distribution. Today, herbal compounds are highly regarded in order to inactivate hydatid cyst protoscoleces. This study aimed to compare the scolicidal activity of hydroalcoholic extract of Pulicaria gnaphalodes and Alhagi maurorum against hydatid cyst protoscoleces in vitro. Methods: The scolicidal activity of P. gnaphalodes and A. maurorum extracts were evaluated at ۵۰, ۱۰۰, ۱۵۰, and ۲۰۰ mg/mL concentrations following ۱۵, ۳۰, and ۶۰ minutes of exposure. Then, they were compared with Albendazole (۵ g/۱۰۰ mL) as positive control and distilled water as negative one in similar doses. The viability of protoscoleces was confirmed with a ۰.۱% eosin stain test under a light microscope. The experiments were performed twice, and data were analyzed by GraphPad software version ۵.۰. Results: The results of this study indicated that P. gnaphalodes extract killed ۱۰۰% of the protoscoleces at a concentration of ۲۰۰ mg/mL after ۳۰ minutes of exposure, but the hydroalcoholic extract of A. maurorum at the same concentration and time could kill ۹۰% of protoscoleces. Conclusion: The findings of the present study confirmed that P. gnaphalodes had a strong scolicidal effect; however; in vivo studies are needed to evaluate the effectiveness of P. gnaphalodes plant.

کلیدواژه ها:

Hydatid cyst ، Scolicidal ، Pulicaria gnaphalodes ، Alhagi maurorum ، In vitro