Isolation and Identification of Salmonella Typhimurium in Retail Meat Sources by Multiplex Polymerase Chain Reaction
محل انتشار: میکروبیولوژی بالینی و عفونت، دوره: 10، شماره: 1
سال انتشار: 1402
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 100
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شناسه ملی سند علمی:
JR_JCMI-10-1_003
تاریخ نمایه سازی: 14 آذر 1402
چکیده مقاله:
Background: Salmonella enterica serovar Typhimurium is considered one of the most important emerging
food-borne pathogens in public health worldwide. Meat is commonly known as the food sources
responsible for the salmonellosis outbreak.
Methods: Overall, ۱۴۱ different meat samples were randomly collected from local markets. The
conventional culture method was performed to isolate Salmonella spp., and then, using two pairs of
primers, a multiplex polymerase chain reaction (PCR) assay was employed to confirm the identification of
isolated colonies as Salmonella spp. and determine serovars as Typhimurium.
Results: Out of ۱۴۱ samples, ۴۸ (۳۴%) ones were presumptively isolated as Salmonella on the Salmonella
agar medium and distributed as ۲۴%, ۲۳%, and ۴۲% among veal, lamb, and chicken meat, respectively.
However, the results of multiplex PCR showed that ۴.۹% of chicken meat was merely identified as S.
Typhimurium. In general, S. Typhimurium isolates were found only in chicken meat.
Conclusion: Salmonella Typhimurium isolates were only observed in the chicken meat. Multiplex PCR was
found to be a specific and rapid alternative method for the identification of various types of Salmonella.
کلیدواژه ها:
نویسندگان
Mazen Safi
Department of Molecular Biology and Biotechnology, Atomic Energy Commission of Syria, Damascus, Syria
Bassam Al Balaa
Department of Molecular Biology and Biotechnology, Atomic Energy Commission of Syria, Damascus, Syria
Samah Qasem
Department of Molecular Biology and Biotechnology, Atomic Energy Commission of Syria, Damascus, Syria
Lailla Al Hallab
Department of Molecular Biology and Biotechnology, Atomic Energy Commission of Syria, Damascus, Syria