Determination of manganese in rat blood samples based on ionic liquid-liquid extraction and chelation therapy for evaluation of manganese toxicity in rats
سال انتشار: 1400
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 94
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شناسه ملی سند علمی:
JR_AMECJ-4-4_005
تاریخ نمایه سازی: 14 آذر 1402
چکیده مقاله:
In this study, the manganese ions were extracted in the blood of rats based
on desferrioxamine (DFO), deferasirox (DFX) and deferiprone (DFP)
as chelators (ligands) by ionic liquid-liquid phase extraction method
(ILLEM) before being determined by F-AAS. Also, the toxic effects of
manganese on blood serum and hematology parameters such as: RBC,
WBC, HGB, PLT and HCT were investigated. Male Wistar rats were
randomly divided into control and toxic groups. Manganese chloride
was administrated orally in low and high doses. Orally (deferasirox and
deferiprone) or intraperitoneally (desferrioxamine) for ۲ weeks. Results
showed that exposure to manganese significantly increased both counts
of hematology parameters and concentration of this metal ion in serum
compared to the control group. By procedure, manganese was chelated
with ligands in the blood of rats and then the hydrophobic ionic liquid
(IL, [HMIM][PF۶]) was added to blood samples. After shaking and
centrifuging, the upper liquid phase was separated by an auto-sampler
and manganese loaded in a mixture of IL/ligand was settled down in the
bottom of the conical tube. The manganese ions were back-extracted
from IL phase and the remained acid solution was determined with
F-AAS. The linear range, LOD and enrichment factor for ۱۰ mL of blood
rats were obtained ۲۵- ۱۸۰ μg L-۱, ۶.۵ μg L-۱ and ۱۹.۹۲, respectively.
By chelation therapy extra manganese ions were removed from human
serum and the normal hematology parameters were achieved.
کلیدواژه ها:
Chelators ، Red blood cells ، Toxicity ، Ionic liquid-liquid extraction ، Flame atomic absorption spectrometry
نویسندگان
S Jamilaldin Fatemi
Department of Chemistry, Shahid Bahonar University of Kerman, Kerman, Iran
Tayyebeh Zandevakili
Department of Chemistry, Shahid Bahonar University of Kerman, Kerman, Iran
Fatemeh Khajoee Nejad
Department of Chemistry, Shahid Bahonar University of Kerman, Kerman, Iran
Marziyeh Iranmanesh
Department of Chemistry, Kerman Branch, Islamic Azad University, Kerman, Iran