Ionic liquid functionlized on multiwall carbon nanotubes for nickel and lead determination in human serum and urine samples by micro solid-phase extraction

In this study, a novel synthesis adsorbent, ۱-(۳-aminopropyl)-۳- methylimidazolium hexafluorophosphate functionlized on multiwall carbon nanotubes ([Apmim][PF۶]-MWCNTs, IL@MWCNTS) was used for nickel/lead (Ni/Pb) extraction and determination by dispersive ionic liquid micro solid-phase extraction (DIL-μ-SPE) coupled to electrothermal atomic absorption spectrometry (ET-AAS). After dilution of ۲۰ mg of IL@MWCNTS in ۲۰۰ μL of acetone, the mixture was injected to ۱۰ mL of human serum/urine samples at pH of ۸.۰. After sonication for ۵ min, the Ni(II) / Pb(II) were extracted by ionic liquid phase and then centrifuged for ۲.۵ min. The upper liquid phase set aside and Ni(II) / Pb(II) loaded in adsorbent were backextracted by acidic solution at pH=۲-۳. Finally, the concentration of total nickel and lead was determined by ET-AAS. By optimizing, the limit of detection, linear range, and enrichment factor for nickel and lead were obtained (۰.۰۵ μg L−۱; ۰.۱ μg L−۱), (۰.۲-۵.۸ μg L−۱; ۰.۴- ۳۰ μg L−۱) and ۲۴.۷; ۵.۱, respectively (RSD less than ۵%). Also, the capacity absorption of IL@MWCNTS for nickel and lead ions were achieved ۱۴۹.۳ mg g-۱ and ۱۶۲.۵ mg g-۱, respectively. The DIL-μ-SPE procedure was validated for nickel and lead extraction by spiking of real samples and ICP-MS analyzer.

کلیدواژه ها:

Nickel and lead ، Human samples ، Ionic liquid ، Multiwall carbon nanotubes ، Micro solid-phase extraction