Identification of Mucorales isolates from soil using morphological and molecular methods

سال انتشار: 1395
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 61

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شناسه ملی سند علمی:

JR_CUMM-2-1_003

تاریخ نمایه سازی: 11 آذر 1402

چکیده مقاله:

Background and Purpose: Soil is the main habitat of saprophytic and pathogenic fungi. Mucormycetes are one of the most parts of soil fungi and certain members are among opportunistic fungi and can cause systemic fungal infections in immunocompromised patients. The majority of human and animal infections are caused by members of the genera Rhizopus, Mucor, Rhizomucor, Lichtheimia (Absidia), Cunninghamella and Mortierella. The objective of this research was to isolate and identify the main genera of Zygomycetes, using molecular assay and morphological features. Materials and Methods: A total of ۳۴۰ soil samples were collected from different sites of seven public parks and ۱۴ municipality districts in Isfahan. All samples were cultured on appropriate media and incubated at ۲۷° C for ۲ to ۴ days, and then examined daily for visible fungal growth. PCR-RFLP method and macroscopic, microscopic and physiological characteristics were applied to identify fungal colonies. Results: Four hundred pure colonies belonging to six genera of Zygomycetes including Lichtheimia, Rhizopus, Rhizomucor, Mucor, Cunninghamella and Mortierella were identified. The genus Rhizopus (۳۵.۵%) was the most frequent isolate, followed by Mucor (۳۲.۲۵%) and Rhizomucor (۲۷.۵%). Conclusion: These finding may help us to understand about the importance of opportunistic fungi in public areas and the risk of exposure with immunocompromised persons.

نویسندگان

Ardeshir Ziaee

Department of Medical and Veterinary Mycology, Faculty of Veterinary Specialized Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran

Mohammadali Zia

Department of Basic Sciences, Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan, Iran

Mansour Bayat

Department of Medical and Veterinary Mycology, Faculty of Veterinary Specialized Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran

Jamal Hashemi

Department of Mycology and Parasitology, Faculty of Public Health, Tehran University of Medical Sciences, Tehran, Iran