Microalgae are valuable organisms due to their ability to produce various beneficial productsfor human utilization [۱].Understanding the interface interactions between microalgae cells andmagnetic nanoparticles (MNPs) is essential for effective magnetic harvesting of microalgae usingMNPs.In order to make large-scale microalgae cultivation economically viable, it is crucial toenhance the harvesting process. Magnetic separation is a straightforward process involving the useof a magnetic separator to remove magnetic contaminants or selective magnetic adsorbents for therecovery of desired products [۲].Magnetic nanoparticles show great potential as they caneffectively attract and bind to microalgal cells, facilitating their separation through a magnetic fieldand enabling efficient harvesting. This technology holds promise for streamlining microalgaeproduction and expanding its applications in various industries. This method enables rapid andcost-effective harvesting of microalgae for various industrial purposes. magnetic nanoparticlesconsisting of Fe۳O۴ were synthesized using the co-precipitation. The researchers utilized thesurface response method (RSM) in conjunction with a central composite design (CCD) tothoroughly investigate the impact of three key factors: time, nanoparticle concentration, and pH inthe culture medium. RSM, a statistical technique, enabled the simultaneous evaluation andoptimization of multiple variables, providing a comprehensive understanding of theirinteractions.These significant findings highlight the effectiveness and feasibility of the magneticnanoparticle-based harvesting method [۳]. Moreover, the utilization of
municipal wastewater as acultivation medium offers significant advantages in terms of cost-effectiveness and sustainability.The use of magnetic nanoparticles and the application of RSM with CCD led to the optimizationof harvesting conditions for native microalgae Chlorella sorokiniana.pa۹۱. The achievedharvesting efficiency of ۷۹% under the optimized conditions demonstrates the efficacy of themagnetic nanoparticle-based method and sets the stage for economic and efficient microalgaeharvesting techniques