Synthesis of Fe-MOF nanostructure: A Study of Peroxidase-Like behaviorfor Sensing of H۲O۲

In recent years, nanotechnology is widely used in sensing, biomedicine, food industry,agriculture and etc. as a newly developed science and technology, in which nanomaterials withenzyme-like catalytic activity, namely nanozyme, have been used in the development of newchemosensors [۱]. Recently, nanozymes have drawn extensively studied for their applications inbioanalysis, disease diagnosis, and therapy. In addition to, compared with natural enzymes,nanozymes possess the advantages of low cost, easy-to-scale production, high tolerance to theenvironment, long-term storage, high stability, and controllability [۲]. Studies in this field haveshown that nanozymes are suitable substitutes for natural enzymes. Therefore, the development ofstable materials exhibiting high substrate affinity and high activity as ideal artificial enzymes is ahighly desirable research goal [۳]. The nanozymes reported thus far are, for instance, noble metalbased,and carbon-based materials, metals/metal oxides, carbon materials, MOFs, and so on.Among these nanomaterials, MOF nanozymes due to their inherent porous structure, large specificsurface area, low density, high porosity, structural diversity, and adjustable pore size open structurefor the free transfer of substances and products in the catalytic reaction, have shown specialenzyme-like activity [۴]. Due to availability effective active sites and enhancing the catalyticactivity in MOFs, it has been reported that some of them can simulate natural enzyme activitiessuch as POD-like (peroxidase) activity. Currently, Due to the same Fe metal centers as peroxidasefor MIL-(Fe) peroxidase-like nanozymes (such as MIL-۵۳, MIL-۸۸, MIL-۱۰۰ and MIL-۱۰۱), thenanozymes based MIL-(Fe) have become a potential platform for exploring catalytic mechanismsand improving performances [۵]. Herein, we reported a Fe-MOF with excellent properties andperoxidase-like activity, which is synthesized with a simple method. The synthesized Fe-MOFunder weakly acidic conditions, can catalyze the oxidation of TMB substrate to oxTMB byhydrogen peroxide. Therefore, a simple colorimetric assay for the detection of hydrogen peroxidebased on the peroxidase-like activity of the Fe-MOF nanozyme has been established.

کلیدواژه ها:

Nanozyme ، Fe-MOF ، Peroxidase